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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be noticed in several tumors, which results in chromosome instability, and thus may possibly lead to tumorigenesis. This protein was discovered to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant purpose in endorsing mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two added splice variants ended up noted but specific nucleotide sequence data is just not however accessible.
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Blocking peptides are peptides that bind precisely to your focus on antibody and block antibody binding. These peptide typically includes the epitope recognized via the antibody.
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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to ensure the best possible outcomes are...
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This manual outlines additional probable optimization of cellular and immunoassay parameters to be certain the best possible results are...
Antibodies sure to the blocking peptide no more bind into the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs into the really conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add for the temporal coordination of each mitotic function. Cyclin E types a complex with and capabilities as being a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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